Operation, scope of applicability, and treatment principle of ozone based autologous blood therapy


Generally speaking, autologous blood therapy, also known as ozone autologous blood therapy, autologous blood transfusion therapy, and ozone immunotherapy. Ozone autologous blood therapy is divided into ozone large autologous blood therapy and ozone small autologous blood therapy. Simply put, it is a method of treating certain diseases by extracting the patient's own blood from the veins, mixing it with ozone, and then reinfusing it back into the patient's body to stimulate non-specific immune responses, promote white blood cell phagocytosis, regulate the body's internal environment, reduce sensitivity, and enhance immunity.
Scope and indications of autologous blood therapy:
The current scope of treatment in clinical practice or research includes:
Atherosclerosis
Cerebral infarction and its sequelae
Hyperlipidemia, hypertension
Gout and lymphedema
Peripheral vascular disease
Geriatric diseases
Peripheral and cerebral blood flow disorders
Stroke prevention and treatment
Symptoms of coronary blood flow disorders during the prevention and treatment of myocardial infarction
Acute and chronic hepatitis
Regulatory therapy
Lesion excitation
Repair therapy
Rheumatic disorders
Malignant tumors, accompanied by radiation therapy
chronic disease
Cardiovascular and cerebrovascular diseases
AIDS, etc
Parkinson's disease
This therapy has varying degrees of efficacy in treating dermatological diseases such as vitiligo, chronic urticaria, systemic pruritus, generalized eczema and dermatitis, allergic purpura, certain bullous diseases, psoriasis, recurrent boils and folliculitis, skin scratch syndrome, chronic eczema, recurrent boils, folliculitis, and purulent acne.
The treatment principle of autologous blood therapy:

So what is the specific treatment method of autologous blood therapy?
The attending physician draws the patient's own blood into a disposable autologous blood bag. Inject medical ozone (100ml, with a ratio of 1:1 between ozone and blood), and finally use a dedicated disposable ozone autologous blood transfusion device to transfer the blood into the body. The strong oxidizing properties of ozone rapidly eliminate bacteria, fungi, or viruses in inflammatory tissues or infection foci within the body; Harmful blood lipids and cholesterol. Triglycerides, low-density lipoprotein, as well as waste products produced by metabolism in the body and toxins secreted by bacteria and viruses, various painful substances, are difficult to escape the fate of being inactivated or oxidized by ozone. Thus playing a role in treating diseases.
Specifically, it refers to the "three activations", "four clearances", and five improvements:
Three activations
Activate red blood cell metabolism, increase hemoglobin oxygen saturation, and enhance tissue utilization of oxygen and ATP;
Activating the body's immune system can increase white blood cells in the blood, enhance phagocytic ability, increase T lymphocytes by 204%, and increase T active lymphocytes by 70%;
Activating the antioxidant enzyme system, increasing the level of 6-ketoprostaglandin F1 in plasma, enhancing the activity of antioxidant enzymes in the body, and reducing the damage of free radicals to the body.
Four clearances
Clearing glucose toxins and their metabolic waste from the blood, enhancing the activity of antioxidant enzymes in the body, and reducing the damage of free radicals to the body.
It can eliminate the lipotoxin and its metabolic waste in the blood, improve the blood viscosity, strengthen the decomposition of cholesterol and triglyceride and reach the standard, eliminate the hidden damage of lipotoxin to the body, prevent the formation of vascular plaque, and prevent systemic atherosclerosis and neuropathy.
Eliminate waste generated by blood metabolism, such as excess lactate, uric acid, acetone, inorganic salts, etc., increase tissue oxygen supply, improve blood quality and cell vitality.
Clearing pathogenic, viral, and toxic cells from the blood: Clearing toxic cells from the blood, inducing cancer cell death, and reducing the risk of cancer.
Five improvements
Improve blood rheology, increase eosinophils in the blood, increase free heparin levels in the blood, reduce red blood cell aggregation, and enhance deformability;
Improve microcirculation, reduce brain edema, and regulate the functional status of cerebral blood vessels;
Improving blood metabolism can lower blood sugar, uric acid, bilirubin, lactate, pyruvic acid, reduce lipid peroxides malondialdehyde (MDA) and thromboxane B2 (TXB2), kill various bacteria, and enhance the body's own immunity;
Improving the condition of the blood vessel wall, reducing platelet aggregation, and decreasing blood viscosity, thereby improving the condition of the blood vessel wall.
Improve oxygen supply, promote blood circulation, enhance cell vitality, and repair nerve cells.

Operating procedures for autologous blood therapy: Treatment steps:


The nurse takes 100ml of venous blood from the patient's elbow and compares it with a pre-set O3 concentration of 40 μ g/ml. Based on the patient's actual situation, the theoretical concentration should not exceed 60 μ g/ml, and the general treatment concentration should not exceed 40 μ g/ml. The focus should be on the patient's comfort. Older people should reduce the concentration appropriately, starting from a low concentration and gradually increasing it Mix in a 1:1 ratio, slowly mix for about 5 minutes until the blood has been thoroughly oxidized, and finally use about 15 minutes (within 25 minutes, not too fast) to return the blood to the patient's body. Da Zi Xue therapy is administered once every 2 days, 3 times a week. The treatment plan varies depending on the specific disease
Standard Operating Procedure
Preparation before treatment
(1) Open the windows (doors) of the treatment room for ventilation; Check the power supply, oxygen cylinder, and interface connections
correct.
(2) Turn on the oxygen cylinder switch, check and ensure there is no air leakage.
(3) Confirm that the power switch of the ozone generator is turned on.
(4) Inspection supplies; Ozone autologous blood therapy specialized consumables (antioxidant reaction bag, blood collection and transfusion device, specialized 50ml syringe), 250ml physiological saline bottle filter converter, treatment vehicle (tourniquet, disinfectant swab, and disinfectant infusion supplies).
2. Prepare ozone reaction bag
(1) Prepare 250ml of 0.9% saline solution and disinfect after opening the lid
(2) Open the blood transfusion device, close all pathways, and insert one end of the long tube into a physiological saline bottle
(3) Open the saltwater pathway, including clamp 1, clamp 3, and flow rate regulator A. Exhaust normally to ensure that the liquid level in the Murphy dropper is not high at 1/2 to prevent the drip rate from being invisible. After confirming that there are no bubbles, close clamp 1, clamp 3, and flow rate regulator A;
(4) Open one end of the short tube of the blood transfusion device, insert it into the blood bag, and open the No. 2 and flow rate regulator B on the same route as the blood bag to discharge 25ml of anticoagulant from the blood bag. At the same time, it also plays a role in flushing the tube, making the entire tube contain anticoagulant.
3. Patient preparation
(1) Select a puncture vein, tie a tourniquet, disinfect the skin, use a specially equipped puncture needle for vein puncture, and fix it.
(2) Open the infusion set regulator, lower the blood bag in the treatment tray, gently shake the blood bag to mix the blood with the anticoagulant, and slowly collect 100ml of blood.
(3) Release the tourniquet, stop blood collection, and switch to intravenous infusion of physiological saline.
4. Ozone extraction operation
(1) Lightly press the ozone concentration 0-80ug/ml button to select the desired ozone concentration.
(2) Prepare a specialized 50ml syringe (without needle), pull the injection piston, and then return it to the zero degree mark.
(3) Insert the syringe head vertically into the large mouth of the conversion filter, and insert the small mouth of the conversion filter into the ozone intake port. Rotate clockwise and hear a "click" sound. After 6 seconds, see the green light flashing on the far right side of the machine. Press and hold this button to slowly lift the syringe with ozone gas to 50 degrees Celsius, and take 100 milliliters of gas.
(4) Carefully and slowly lift the syringe vertically with both hands, detach it from the ozone intake port, and immediately reverse the syringe to make the port face upwards.
5. Blood ozone mixing operation
(1) Connect the blood bag injection port to the ozone injector and open clamp 4.
(2) Inject 100ml of ozone gas into the blood bag, and after injection, close clamp 4.
6. Blood transfusion
(1) Hang the blood bag on the infusion rack, stop the infusion of physiological saline, and let the blood flow back into the patient's body.
(2) After there is no blood in the blood bag, open the saline flushing tube and remove the puncture needle; Advise the patient to stop bleeding and rest for 10 minutes, and discard the blood transfusion (liquid) device in the contaminated trash can (yellow).
7. Zeroing
After completing all treatments, close the oxygen valve and return the oxygen pressure gauge to zero.

Precautions for Ozone Autohemotherapy
1. Due to the fact that ozone autologous blood therapy is a clinical treatment technique that integrates blood collection, transfusion, and mixed blood, strict adherence to aseptic techniques and verification systems should be implemented.
2. The amount of blood extracted must match the amount of anticoagulant drugs. Too little anticoagulant may cause blood clotting, while too much anticoagulant can harm physical health.
3. When reintroducing blood into the patient's body, start slowly (40-60 drops) and gradually increase the infusion rate (approximately 10-15 minutes to complete). High pressure transfusion (200ml of blood collected within 3 minutes and 200ml of blood rapidly transfused within 4 minutes) may cause air embolism, induce or worsen heart failure, and other unsafe factors. It is not recommended to use this therapy, and it is recommended to use atmospheric ozone autologous blood therapy.
4. Pure oxygen must be used as the raw material. After taking ozone gas from the syringe, the tip of the syringe should be facing upwards and no air should be mixed in, otherwise it will produce toxic nitrite gas to the human body; The concentration of ozone generated in the first two tubes during each ozone gas treatment may not be accurate, and it may be reduced by injecting it into the machine.
5. Medical ozone is a mixed gas of ozone and medical pure oxygen. If the therapeutic concentration is too high, it can cause harm to the body, while if the concentration is too low, it becomes a "placebo". Therefore, it is necessary to accurately determine the dosage of medical ozone. According to "Ozone Therapy", clinical trials, and the application effects of major hospitals in China, the suitable therapeutic concentration for ozone autologous blood therapy is currently 20ug/ml-40ug/ml, and the highest ozone concentration for autologous blood therapy cannot exceed 50ug/ml. The first treatment starts from a low concentration and gradually increases the ozone concentration.
6. When blood and ozone are mixed, the movements should be gentle to avoid violent shaking that can cause the destruction of red blood cells in the blood and reduce the effectiveness of treatment; The mixing time is about 5 minutes, as it is clearly stated in "Ozone Therapy" (Chapter 9: Understanding the Basic Knowledge of Ozone Activity and Potential Risks of Ozone Therapy) that ozone mixed with blood produces trace amounts of harmful substances to the body. In order to avoid the formation of harmful concentration gradients, blood oxidation must be carried out continuously and gently mixed for at least 5 minutes.
7. It is strictly forbidden to directly inject medical ozone mixed gas into blood vessels during operation, as this method may cause air embolism and endanger life.
8. Strictly implement aseptic operations and the three checks and seven pairs system to avoid errors such as blood contamination or incorrect blood transfusion.
9. During ozone therapy, it is necessary to suspend the use of all antioxidant supplements containing vitamin C and vitamin E. The high concentration of these compounds in the blood can interfere with the effectiveness of ozone as an oxidant. Therefore, vitamins or antioxidants should be used before or after ozone therapy, but not during the treatment process.
10. Ozone gas extraction should be strictly carried out according to standard operating procedures, keeping the therapeutic device clean. It is strictly prohibited to operate the device with contaminated gloves, especially paying attention to the cleanliness and protection of the gas extraction port; It is strictly prohibited to clean the air intake with liquid.
11. The indoor environment should be clean and quiet, with good ventilation. Smoking or the use of open flames is strictly prohibited; It is strictly prohibited to use flammable and explosive gases such as ether and ethylene oxide in the treatment room; Do not use peracetic acid spray to disinfect the indoor air.
Precautions before, during, and after autologous blood therapy treatment:

1. Before starting each ozone blood therapy treatment, the ozone therapy device should be turned on to check if it is working properly.
2. Check the quality, labels, expiration dates, batch numbers, etc. of the drugs, infusion devices, blood bags, and syringes required for ozone autotransfusion therapy.
4. Provide psychological care for patients, introduce the operation process of autologous blood therapy and possible reactions during treatment, answer patients' questions, make patients mentally prepared, eliminate fear, and encourage them to actively cooperate with treatment.
5. Measure body temperature, pulse, respiration, and blood pressure before treatment, understand the overall condition, and advise the patient to drink more water to dilute the blood.
6. Select the appropriate puncture vessel (the most commonly used site is the area in front of the elbow with abundant blood vessels and close to the skin surface), and observe whether there is redness, swelling, or rupture at the puncture site.
7. Exhaust: Rinse the tube with physiological saline to eliminate the air inside the infusion tube, preventing the air inside the tube from entering the body during blood transfusion and causing air embolism.
8. Disinfection: With the puncture point as the center, use a cotton swab dipped in iodine to apply it in a spiral from the inside out, and then use 75% alcohol to remove iodine. The disinfection range is about 3cm in diameter, and be careful not to reapply to the disinfected area.
9. Puncture personnel should have proficient puncture techniques to avoid multiple punctures, which can cause physical pain to patients and increase their psychological burden; Multiple punctures at the same time can cause mechanical damage to blood vessels, affecting future treatment.
10. When collecting blood, tighten your fists and tie the tourniquet tightly. Do not move your arms casually and secure the needle to ensure that the needle does not move.
11. When mixing blood with ozone, the movement should be gentle to avoid violent shaking that may cause artificial destruction of red blood cells in the blood. The mixing time is 5 minutes, as Chapter 9 of "Ozone Therapy" (Understanding the Basic Knowledge of Ozone Activity and Potential Risks of Ozone Therapy) clearly states that ozone mixed with blood produces trace amounts of harmful substances to the body. In order to avoid the formation of harmful concentration gradients, blood oxidation must be carried out continuously and gently mixed for at least 5 minutes.
12. Pay attention to observation: During the entire treatment process (blood collection, blood transfusion), closely observe the patient's demeanor and facial expressions, and repeatedly ask if the patient is feeling unwell.
13. After the treatment is completed and the needle is removed, let the patient rest on the bed for a while and press the needle hole correctly for 3-5 minutes to prevent hematoma, congestion, or adhesion between blood vessels and surrounding tissues.
Side effects and treatment methods of ozone autologous blood therapy

1. The occurrence of chest urgency, tachycardia, shortness of breath, cyanosis, and blood pressure drop during ozone autologous blood therapy is caused by excessive cardiac load. This reaction often occurs during treatment due to heart dysfunction and rapid blood transfusion. In this regard, the speed of reinfusion should be strictly controlled during treatment. For patients with heart dysfunction and heart failure, a drip rate of no more than 20-40 drops per minute can prevent this reaction from occurring (high-pressure ozone therapy is most likely to cause this symptom and is currently prohibited from use. It is recommended to use atmospheric pressure ozone autologous blood therapy).
2. Dizziness, chest discomfort, and pain behind the sternum are manifestations of air embolism during the treatment process, which can be life-threatening in severe cases. The main reason is that the air in the infusion tube is not completely discharged and the connection of the blood transfusion device is not tight. As long as we carefully check whether the connection parts of the blood transfusion device are tight, pay attention to expelling the internal air before puncture, and strengthen inspections during treatment, we can prevent such situations from happening.
3. Febrile reactions often occur 1-2 hours after the end of treatment, due to various reasons, including endogenous heat sources and external heat sources. Symptoms include headache, chills, chills, fever, and overall discomfort. We pay special attention to aseptic operations and store various therapeutic supplies in the purification table for safekeeping. If there is a fever reaction, timely symptomatic care should be taken to slow down the blood transfusion speed, and in severe cases, the transfusion should be stopped; Those who are afraid of cold and chills should receive treatment such as insulation. For those with a fever above 38 ℃, physical cooling should be given, and if necessary, cooling drugs, antibiotics, sedatives, and other treatments should be given according to medical advice.
4. Lips and tongue pain (common in women), most commonly occurring near the end of autologous blood transfusion, may be caused by transient, mild hypocalcemia due to excessive accumulation of citrate. When this symptom occurs, calcium supplementation treatment should be given, and attention should be paid to the self blood therapy course, 2-3 days/time. During treatment, the blood and sodium citrate should be placed in proportion.
5. After treatment, some patients feel tired, some patients have no symptoms, and some patients feel healthy and energetic. Why did this situation occur? This is mainly due to differences in the antioxidant system capacity within each individual's body, so ozone autologous blood therapy (especially for the elderly) must start with a low ozone dose (20ug/ml) and slowly increase to 40-50 ug/ml. If the above self blood therapy is effective, several courses of injections can be administered according to the condition to consolidate the therapeutic effect.





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